No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis (2024)

Schrijver, B., Hardjosantoso, H., Ten Berge, J. C. E. M., Schreurs, M. W. J., Van Hagen, P. M., Brooimans, R. A., Rothova, A. (2021). No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis. Ocular Immunology and Inflammation, 29(5), 883-889. https://doi.org/10.1080/09273948.2019.1698752

Schrijver, Benjamin ; Hardjosantoso, Hannah ; Ten Berge, Josianne C E M et al. / No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis. In: Ocular Immunology and Inflammation. 2021 ; Vol. 29, No. 5. pp. 883-889.

@article{40f8620668334e1a9ccfe4435ab461cf,

title = "No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis",

abstract = "Purpose: To detect circulating retina-specific autoreactive CD4+ T-cells and antiretinal antibodies (ARA) in latent tuberculosis (TB)-associated uveitis or sarcoid uveitis patients.Methods: The presence of crude retinal extract (RE) autoreactive CD4+ T-cells was determined by a highly sensitive flowcytometric-based technique examining co-expression of CD25 and CD134 (OX40) on RE stimulated PBMC. The presence of ARA in available matched serum samples was assessed by indirect immunofluorescence.Results: No autoreactive CD4+ T-cells against RE could be detected in either latent TB-associated uveitis or sarcoid uveitis patients, while ARA were detected in the serum of the majority (5/6) of latent TB-associated uveitis and all (3/3) sarcoid uveitis patients.Conclusion: Even with the use of this highly sensitive flowcytometric technique circulating retina-specific autoreactive CD4+ T-cells could not be detected. In contrast, ARA were detected in the majority of patients indicating an adaptive humoral immune response toward retinal antigens had occurred.",

author = "Benjamin Schrijver and Hannah Hardjosantoso and {Ten Berge}, {Josianne C E M} and Schreurs, {Marco W J} and {Van Hagen}, {P Martin} and Brooimans, {Rik A} and Aniki Rothova and Dik, {Willem A}",

note = "{\textcopyright} 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.",

year = "2021",

month = jul,

day = "4",

doi = "10.1080/09273948.2019.1698752",

language = "English",

volume = "29",

pages = "883--889",

journal = "Ocular Immunology and Inflammation",

issn = "0927-3948",

publisher = "Taylor & Francis Ltd",

number = "5",

}

Schrijver, B, Hardjosantoso, H, Ten Berge, JCEM, Schreurs, MWJ, Van Hagen, PM, Brooimans, RA, Rothova, A 2021, 'No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis', Ocular Immunology and Inflammation, vol. 29, no. 5, pp. 883-889. https://doi.org/10.1080/09273948.2019.1698752

No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis. / Schrijver, Benjamin; Hardjosantoso, Hannah; Ten Berge, Josianne C E M et al.
In: Ocular Immunology and Inflammation, Vol. 29, No. 5, 04.07.2021, p. 883-889.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis

AU - Schrijver, Benjamin

AU - Hardjosantoso, Hannah

AU - Ten Berge, Josianne C E M

AU - Schreurs, Marco W J

AU - Van Hagen, P Martin

AU - Brooimans, Rik A

AU - Rothova, Aniki

AU - Dik, Willem A

N1 - © 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

PY - 2021/7/4

Y1 - 2021/7/4

N2 - Purpose: To detect circulating retina-specific autoreactive CD4+ T-cells and antiretinal antibodies (ARA) in latent tuberculosis (TB)-associated uveitis or sarcoid uveitis patients.Methods: The presence of crude retinal extract (RE) autoreactive CD4+ T-cells was determined by a highly sensitive flowcytometric-based technique examining co-expression of CD25 and CD134 (OX40) on RE stimulated PBMC. The presence of ARA in available matched serum samples was assessed by indirect immunofluorescence.Results: No autoreactive CD4+ T-cells against RE could be detected in either latent TB-associated uveitis or sarcoid uveitis patients, while ARA were detected in the serum of the majority (5/6) of latent TB-associated uveitis and all (3/3) sarcoid uveitis patients.Conclusion: Even with the use of this highly sensitive flowcytometric technique circulating retina-specific autoreactive CD4+ T-cells could not be detected. In contrast, ARA were detected in the majority of patients indicating an adaptive humoral immune response toward retinal antigens had occurred.

AB - Purpose: To detect circulating retina-specific autoreactive CD4+ T-cells and antiretinal antibodies (ARA) in latent tuberculosis (TB)-associated uveitis or sarcoid uveitis patients.Methods: The presence of crude retinal extract (RE) autoreactive CD4+ T-cells was determined by a highly sensitive flowcytometric-based technique examining co-expression of CD25 and CD134 (OX40) on RE stimulated PBMC. The presence of ARA in available matched serum samples was assessed by indirect immunofluorescence.Results: No autoreactive CD4+ T-cells against RE could be detected in either latent TB-associated uveitis or sarcoid uveitis patients, while ARA were detected in the serum of the majority (5/6) of latent TB-associated uveitis and all (3/3) sarcoid uveitis patients.Conclusion: Even with the use of this highly sensitive flowcytometric technique circulating retina-specific autoreactive CD4+ T-cells could not be detected. In contrast, ARA were detected in the majority of patients indicating an adaptive humoral immune response toward retinal antigens had occurred.

U2 - 10.1080/09273948.2019.1698752

DO - 10.1080/09273948.2019.1698752

M3 - Article

C2 - 31913737

SN - 0927-3948

VL - 29

SP - 883

EP - 889

JO - Ocular Immunology and Inflammation

JF - Ocular Immunology and Inflammation

IS - 5

ER -

Schrijver B, Hardjosantoso H, Ten Berge JCEM, Schreurs MWJ, Van Hagen PM, Brooimans RA et al. No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis. Ocular Immunology and Inflammation. 2021 Jul 4;29(5):883-889. Epub 2020 Jan 8. doi: 10.1080/09273948.2019.1698752